changes in microbial energy metabolism measured by nanocalorimetry during growth phase transitions

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ID: 130464
2018
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Ranked #235 of 875 articles by views in journal of magnetic resonance (san diego, calif : 1997)

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Abstract
Calorimetric measurements of the change in heat due to microbial metabolic activity convey information about the kinetics, as well as the thermodynamics, of all chemical reactions taking place in a cell. Calorimetric measurements of heat production made on bacterial cultures have recorded the energy yields of all co-occurring microbial metabolic reactions, but this is a complex, composite signal that is difficult to interpret. Here we show that nanocalorimetry can be used in combination with enumeration of viable cell counts, oxygen consumption rates, cellular protein content, and thermodynamic calculations to assess catabolic rates of an isolate of Shewanella oneidensis MR-1 and infer what fraction of the chemical energy is assimilated by the culture into biomass and what fraction is dissipated in the form of heat under different limiting conditions. In particular, our results demonstrate that catabolic rates are not necessarily coupled to rates of cell division, but rather, to physiological rearrangements of S. oneidensis MR-1 upon growth phase transitions. In addition, we conclude that the heat released by growing microorganisms can be measured in order to understand the physiochemical nature of the energy transformation and dissipation associated with microbial metabolic activity in conditions approaching those found in natural systems.
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robador2018frontierschanges Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Alberto Robador;Alberto Robador;Alberto Robador;Douglas E. LaRowe;Douglas E. LaRowe;Steven E. Finkel;Steven E. Finkel;Jan P. Amend;Jan P. Amend;Jan P. Amend;Kenneth H. Nealson;Kenneth H. Nealson
Journal journal of magnetic resonance (san diego, calif : 1997)
Year 2018
DOI
10.3389/fmicb.2018.00109
URL
http://journal.frontiersin.org/article/10.3389/fmicb.2018.00109/full
Keywords
energy metabolism calorimetry shewanella oneidensis

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