in vivo flow cytometry of circulating tumor-associated exosomes

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ID: 130085
2016
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Abstract
Circulating tumor cells (CTCs) demonstrated the potential as prognostic markers of metastatic development. However, the incurable metastasis can already be developed at the time of initial diagnosis with the existing CTC assays. Alternatively, tumor-associated particles (CTPs) including exosomes can be a more valuable prognostic marker because they can be released from the primary tumor long before CTCs and in larger amount. However, little progress has been made in high sensitivity detection of CTPs, especially in vivo. We show here that in vivo integrated photoacoustic (PA) and fluorescence flow cytometry (PAFFC) platform can provide the detection of melanoma and breast-cancer-associated single CTPs with endogenously expressed melanin and genetically engineered proteins or exogenous dyes as PA and fluorescent contrast agents. The two-beam, time-of-light PAFFC can measure the sizes of CTCs and CTPs and identify bulk and rolling CTCs and CTC clusters, with no influence on blood flow instability. This technique revealed a higher concentration of CTPs than CTCs at an early cancer stage. Because a single tumor cell can release many CTPs and in vivo PAFFC can examine the whole blood volume, PAFFC diagnostic platform has the potential to dramatically improve (up to 105-fold) the sensitivity of cancer diagnosis.
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nolan2016analyticalin Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Jacqueline Nolan;Mustafa Sarimollaoglu;Dmitry A. Nedosekin;Azemat Jamshidi-Parsian;Ekaterina I. Galanzha;Rajshekhar A. Kore;Robert J. Griffin;Vladimir P. Zharov
Journal oriente moderno
Year 2016
DOI
10.1155/2016/1628057
URL
http://dx.doi.org/10.1155/2016/1628057
Keywords
neoplasms Oncology including cancer and carcinogenscytology tumors

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