seed disinfestation methods for in vitro cultivation of epiphyte orchids from southern brazil métodos de desinfestação de sementes para o cultivo in vitro de orquídeas epífitas do sul do brasil

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ID: 128742
2006
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Abstract
Disinfestation of orchid seeds is necessary when the germination is performed in vitro. In such case, cultures are supplemented by a source of sugar and salts in order to obtain energy and other requirements. However, the presence of sugar and salts easily allows the development of unwanted microorganisms. To avoid such phenomenon, an efficient procedure must be done as a pretreatment of the seeds with liquid or gaseous substances. In this study with several Brazilian orchids, the seeds were disinfested with sodium hypochlorite solutions containing 0.4% or 0.8% active chlorine for five minutes and sodium hypochlorite or formaldehyde fumes up to two hours, this procedure being efficient for such purpose and making it possible to be used routinely.
A desinfestação de sementes de orquídeas é necessária quando a germinação é efetuada in vitro. Neste caso, as culturas são suplementadas por uma fonte de açúcar e de sais, para prover energia e outras necessidades. A presença de açúcares e sais facilmente permite o desenvolvimento de microorganismos indesejáveis. Para evitar estes microorganismos, um procedimento eficiente deve ser usado como pré-tratamento das sementes com substâncias liquidas ou gasosas. Neste ensaio com várias espécies de orquídeas brasileiras, as sementes foram desinfestadas com solução de hipoclorito de sódio 0,4% ou 0,8% de cloro nascente por cinco minutos ou, por vapores de formol ou hipoclorito de sódio por até duas horas, sendo eficientes estes procedimentos para desinfestação e possível seu uso rotineiramente.
Reference Key
alvarez-pardo2006horticulturaseed Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Veronica M Alvarez-Pardo;Alfredo Gui Ferreira;Valter F Nunes
Journal research on biomedical engineering
Year 2006
DOI
10.1590/S0102-05362006000200019
URL
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362006000200019
Keywords
germination in vitro culture

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