A Straightforward Assay for Screening and Quantification of Biosurfactants in Microbial Culture Supernatants.

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ID: 127996
2020
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Ranked #16 of 41 articles by views in Frontiers in bioengineering and biotechnology

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Abstract
A large variety of microorganisms produces biosurfactants with the potential for a number of diverse industrial applications. To identify suitable wild-type or engineered production strains, efficient screening methods are needed, allowing for rapid and reliable quantification of biosurfactants in multiple cultures, preferably at high throughput. To this end, we have established a novel and sensitive assay for the quantification of biosurfactants based on the dye Victoria Pure Blue BO (VPBO). The assay allows the colorimetric assessment of biosurfactants directly in culture supernatants and does not require extraction or concentration procedures. Working ranges were determined for precise quantification of different rhamnolipid biosurfactants; titers in culture supernatants of recombinant KT2440 calculated by this assay were confirmed to be the same ranges detected by independent high-performance liquid chromatography (HPLC)-charged aerosol detector (CAD) analyses. The assay was successfully applied for detection of chemically different anionic or non-ionic biosurfactants including mono- and di-rhamnolipids (glycolipids), mannosylerythritol lipids (MELs, glycolipids), 3-(3-hydroxyalkanoyloxy) alkanoic acids (fatty acid conjugates), serrawettin W1 (lipopeptide), and acyltyrosine (lipoamino acid). In summary, the VPBO assay offers a broad range of applications including the comparative evaluation of different cultivation conditions and high-throughput screening of biosurfactant-producing microbial strains.
Reference Key
kubicki2020afrontiers Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Kubicki, Sonja;Bator, Isabel;Jankowski, Silke;Schipper, Kerstin;Tiso, Till;Feldbrügge, Michael;Blank, Lars M;Thies, Stephan;Jaeger, Karl-Erich;
Journal Frontiers in bioengineering and biotechnology
Year 2020
DOI
10.3389/fbioe.2020.00958
URL
Keywords
Screening quantification colorimetric assay rhamnolipid biosurfactants victoria pure blue bo recombinant production

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