A rapid and simple single-step method for the purification of Toxoplasma gondii tachyzoites and bradyzoites.

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ID: 127993
2020
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Abstract
This study describes a simple method for the large-scale isolation of pure Toxoplasma gondii tachyzoites and bradyzoites. T. gondii tachyzoites were obtained from infected human foreskin fibroblasts (HFFs) and peritoneal exudates of mice, while tissue cysts containing bradyzoites were collected from chronically infected mice. Harvested cells and brain tissues were incubated in Hanks balanced salt solution (HBSS), containing 0.25% trypsin and 0.5% taurodeoxycholic acid (TDC) for 5 min. Subsequent washes in phosphate buffered saline (PBS) were conducted, and the cell viability of the preparations was good, as determined by flow cytometry and ability to reinfect HFF cells and propagate in mice. The purification procedure allowed for a rapid preparation of pure T. gondii tachyzoites and bradyzoites in sufficient quantity that can be used for downstream procedures. The advantage of the new method is that it is convenient and inexpensive.
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elashram2020aveterinary Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors El-Ashram, Saeed;Zhang, Yu;Ji, Yongsheng;Salama, Dina;Mei, Kun;Zhili, Li;Shujian, Huang;Zhang, Haoji;Aboelhadid, Shawky M;Alajmi, Reem A;Metwally, Dina M;El-Khadragy, Manal F;Hargis, Billy M;Tellez-Isaias, Guillermo;Cenci-Goga, Beniamino T;Karama, Musafiri;Marufu, Munyaradzi C;Abouhajer, Fathi;Ali Abdelhafez Hamady, Gamal;El Wakil, Abeer;Al Nasr, Ibrahim;Suo, Xun;
Journal Veterinary medicine and science
Year 2020
DOI
10.1002/vms3.364
URL
Keywords
purification flow cytometry trypsin t. gondii tachyzoites and bradyzoites taurodeoxycholic acid

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