Asymmetric Reprogramming Capacity of Parental Pronuclei in Mouse Zygotes

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ID: 127977
2014
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Abstract
It has been demonstrated that reprogramming factors are sequestered in the pronuclei of zygotes after fertilization, because zygotes enucleated at the M phase instead of interphase of the first mitosis can support the development of cloned embryos. However, the contribution of the parental pronucleus derived from either the sperm or the oocyte in reprogramming remains elusive. Here, we demonstrate that the parental pronuclei have asymmetric reprogramming capacities and that the reprogramming factors reside predominantly in the male pronucleus. As a result, only female pronucleus-depleted (FPD) mouse zygotes can reprogram somatic cells to a pluripotent state and support the full-term development of cloned embryos; male pronucleus-depleted (MPD) zygotes fail to support somatic cell reprogramming. We further demonstrate that fusion of an additional male pronucleus into a zygote greatly enhances reprogramming efficiency. Our data provide a clue to further identify critical reprogramming factors in the male pronucleus.
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liu2014asymmetriccell Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Liu, Wenqiang;Yin, Jiqing;Kou, Xiaochen;Jiang, Yonghua;Gao, Haibo;Zhao, Yanhong;Huang, Bo;He, Wenteng;Wang, Hong;Han, Zhiming;Gao, Shaorong;
Journal Cell reports
Year 2014
DOI
DOI not found
URL
http://www.sciencedirect.com/science/article/pii/S2211124714001181
Keywords
Biology (General) Medicine (General) Medicine Technology Science agriculture general. including nature conservation, geographical distribution

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