Strophalloside Induces Apoptosis of SGC-7901 Cells through the Mitochondrion-Dependent Caspase-3 Pathway

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ID: 118154
2015
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Abstract
Cardenolides with special chemical structures have been considered as effective anti-cancer drugs in clinic trials. Strophalloside is a cardenolide we recently isolated from Antiaris toxicaria obtained from Hainan, China. The aim of this study was to investigate the possible anticancer effects induced by strophalloside and the underlying molecular mechanism. Gastric carcinoma SGC-7901 cells were treated with strophalloside at various concentrations for different times, and resulting cell viability was determined by the MTT assay, and the motility and invasion of tumor cells were assessed by the Transwell chamber assay. Apoptosis were measured by Annexin V-FITC/PI and Hoechst staining. The changes of mitochondrial transmembrane potential were examined by a JC-1 kit. The expressions of pro-apoptotic protein cytochrome c, caspase-3 and caspase-9 were detected by western blotting analysis. The results showed that strophalloside was capable of reducing cell viability, inhibiting cell growth, and suppressing cell migration and invasion in a time- and dose-dependent manner. Mitochondrial membrane potential declined and the concentration of cytochrome c increased in cytoplasm and caspase-3 and caspase-9 were cleaved into activated states, suggesting that cytochrome c was released from the mitochondrion to cytoplasm and finally activated the caspase-dependent apoptosis pathway. Our results indicate that strophalloside is a potential anticancer drug.
Reference Key
dai2015moleculesstrophalloside Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Xue-Jiao Zhang,Wen-Li Mei,Guang-Hong Tan,Cai-Chun Wang,Songlin Zhou,Feng-Ru Huang,Bin Chen,Hao Dai;Xue-Jiao Zhang;Wen-Li Mei;Guang-Hong Tan;Cai-Chun Wang;Songlin Zhou;Feng-Ru Huang;Bin Chen;Hao Dai;
Journal molecules
Year 2015
DOI
10.3390/molecules20045714
URL
https://sciprofiles.com/publication/view/bf6e05e1033a57c13abe77dab728e398
Keywords
apoptosis gastric carcinoma caspase cytochrome c strophalloside mitochondrion-mediated pathway

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