Insights into the Natural Defenses of a Coral Reef Fish Against Gill Ectoparasites: Integrated Metabolome and Microbiome Approach

Clicks: 338
ID: 117612
2020
Free PDF
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal
Emerging

Ranked #12 of 17 articles by views in Metabolites

Most read Least read

Bar heights use a square-root scale.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
Understanding natural defense mechanisms against parasites can be a valuable tool for the development of innovative therapies. We have previously identified a butterflyfish species (Chaetodon lunulatus) that avoids gill monogenean parasites while living amongst closely related parasitized species. The metabolome and microbiome of several sympatric butterflyfish species from the island of Moorea (French Polynesia) were previously described. In this study, we used the previously generated datasets in an attempt to identify metabolites and bacteria potentially involved in parasite defense mechanisms. We investigated the interplay between the gill mucus metabolome and microbiome of the non-susceptible C. lunulatus versus sympatric butterflyfish species that were always found parasitized in the Central and Eastern Indo-Pacific. After observing significant differences between the metabolome and bacteria of susceptible versus non-susceptible fish, we obtained the discriminant metabolites and operational taxonomic units (OTUs) using a supervised analysis. Some of the most important discriminant metabolites were identified as peptides, and three new peptides derived from β-subunit hemoglobin from C. lunulatus (CLHbβ-1, CLHbβ-2, and CLHbβ-3) were purified, characterized and synthesized to confirm their structures. We also identified specific bacterial families and OTUs typical from low-oxygen habitats in C. lunulatus gill mucus. By using a correlation network between the two datasets, we found a Fusobacteriaceae strain exclusively present in C. lunulatus and highly correlated to the peptides. Finally, we discuss the possible involvement of these peptides and Fusobacteriaceae in monogenean avoidance by this fish species.
Reference Key
tapissier‐bontemps2020metabolitesinsights Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Miriam Reverter,P Sasal,Marcelino T. Suzuki,Delphine Raviglione,Nicolas Inguimbert,Alan Pare,Bernard Banaigs,Sébastien N. Voisin,Philippe Bulet,Nathalie Tapissier‐Bontemps;Miriam Reverter;P Sasal;Marcelino T. Suzuki;Delphine Raviglione;Nicolas Inguimbert;Alan Pare;Bernard Banaigs;Sébastien N. Voisin;Philippe Bulet;Nathalie Tapissier‐Bontemps;
Journal Metabolites
Year 2020
DOI
10.3390/metabo10060227
URL
https://sciprofiles.com/publication/view/0ccf404ca9b634d10e4171520bc12e1b
Keywords
metabolites metabolome microbiome gill structures butterflyfish parasitized lunulatus clhbβ

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.