Isolation and Characterization of a Novel Bacterium Bsp-1 Producing Alkaline Lipase.

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ID: 11630
2019
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Abstract
Active lipase-producing bacterium Bps-1 was rapidly isolated using a modified trypan blue and tetracycline, ampicillin (TB-TA) plate. The electro-phoretically pure enzyme was obtained by purification using ethanol precipitation, ion-exchange chromatography, and gel filtration chromatography. The molecular weight was 34.6 kDa and the specific activity was determined to be 443.9 U/mg. The purified lipase showed the highest activity after hydrolysis with p-NPC at a pH of 8.5 and 50°C, and the , , and / values were 1.05, 292.95 s and 279 smM, respectively. The lipase was highly stable at 7.5 ≤ pH ≤ 10.0. K and Na exerted activation effects on the lipase which had favorable tolerance to short-chain alcohols with its residual enzyme activity being 110% after being maintained in 30% ethanol for 1 h. The results demonstrated that the lipase produced by the strain Bps-1 has high enzyme activity and is an alkaline lipase. The lipase has promising chemical properties for a range of applications in the food-processing and detergent industries, and has particularly high potential for use in the manufacture of biodiesel.
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zhu2019isolationjournal Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Zhu, Jing;Liu, Yanjing;Qin, Yan;Pan, Lixia;Li, Yi;Liang, Ge;Wang, Qingyan;
Journal Journal of microbiology and biotechnology
Year 2019
DOI
10.4014/jmb.1903.03045
URL
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