Gaussia Luciferase as a Reporter for Quorum Sensing in Staphylococcus aureus

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ID: 115264
2020
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Abstract
Gaussia luciferase (GLuc) is a secreted protein with significant potential for use as a reporter of gene expression in bacterial pathogenicity studies. To date there are relatively few examples of its use in bacteriology. In this study we show that GLuc can be functionally expressed in the human pathogen Staphylococcus aureus and furthermore show that it can be used as a biosensor for the agr quorum sensing (QS) system which employs autoinducing peptides to control virulence. GLuc was linked to the P3 promoter of the S. aureusagr operon. Biosensor strains were validated by evaluation of chemical agent-mediated activation and inhibition of agr. Use of GLuc enabled quantitative assessment of agr activity. This demonstrates the utility of Gaussia luciferase for in vitro monitoring of agr activation and inhibition.
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Authors Isobel Blower;Carmen Tong;Xiaohui Sun;Ewan Murray;Jeni Luckett;Weng Chan;Paul Williams;Philip Hill;Blower, Isobel;Tong, Carmen;Sun, Xiaohui;Murray, Ewan;Luckett, Jeni;Chan, Weng;Williams, Paul;Hill, Philip;
Journal sensors
Year 2020
DOI
10.3390/s20154305
URL
https://www.mdpi.com/1424-8220/20/15/4305
Keywords
staphylococcus aureus agr quorum sensing gaussia luciferase

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