DNA cytosine methylation and heat-induced deamination

Clicks: 297
ID: 114174
1970
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Abstract
The heat-induced conversion of 5-methylcytosine (m5C) residues to thymine residues and of cytosine to uracil residues in single-stranded DNA was studied. The calculated rates for deamination at 37°C and pH 7.4 were ∼9.5×10−10 and 2.1×10−10 sec−1, respectively. N4-Methyldeoxycytidine, which is in the DNA of certain thermophilic bacteria, was more heat-resistant than was deoxycytidine and much more than was 5-methyldeoxycytidine. Thermophilic bacteria which contain N4-methylcytosine rather than m5C in their genomes may thereby largely avoid heat-induced mutation due to deamination, which is incurred by the many organisms that contain m5C in their DNA.
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Authors Melanie Ehrlich;Kyle F. Norris;Richard Y. Wang;Kenneth C. Kuo;Charles W. Gehrke;Melanie Ehrlich;Kyle F. Norris;Richard Y. Wang;Kenneth C. Kuo;Charles W. Gehrke;
Journal Bioscience reports
Year 1970
DOI
doi:10.1007/BF01116426
URL
https://link.springer.com/article/10.1007/BF01116426
Keywords
Biotechnology biochemistry biomedicine general human genetics

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