DNA cytosine methylation and heat-induced deamination

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ID: 114174
1970
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Abstract
The heat-induced conversion of 5-methylcytosine (m5C) residues to thymine residues and of cytosine to uracil residues in single-stranded DNA was studied. The calculated rates for deamination at 37°C and pH 7.4 were ∼9.5×10−10 and 2.1×10−10 sec−1, respectively. N4-Methyldeoxycytidine, which is in the DNA of certain thermophilic bacteria, was more heat-resistant than was deoxycytidine and much more than was 5-methyldeoxycytidine. Thermophilic bacteria which contain N4-methylcytosine rather than m5C in their genomes may thereby largely avoid heat-induced mutation due to deamination, which is incurred by the many organisms that contain m5C in their DNA.
Reference Key
ehrlich1970biosciencedna Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Melanie Ehrlich;Kyle F. Norris;Richard Y. Wang;Kenneth C. Kuo;Charles W. Gehrke;Melanie Ehrlich;Kyle F. Norris;Richard Y. Wang;Kenneth C. Kuo;Charles W. Gehrke;
Journal Bioscience reports
Year 1970
DOI
doi:10.1007/BF01116426
URL
https://link.springer.com/article/10.1007/BF01116426
Keywords
Biotechnology biochemistry biomedicine general human genetics

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