Fusant Trichoderma HF9 with enhanced extracellular chitinase and protein content

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ID: 112131
2012
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Abstract
Strain improvement was carried out to obtain higher chitinase and protein by inter-specific protoplast fusion between Trichoderma harzianum and Trichoderma viride. Fusant HF9 and parental strains of Trichoderma were compared for chitinase and protein production. 1% of glucose, sucrose and fungal cell wall (Rhizoctonia solani), were used as carbon source for cultivation of Trichoderma and fungal cell wall was the best to induce chitinase and protein. Usage of 0.5% colloidal chitin for the fungal growth under aerated conditions at pH 6.5 and 28°C led to higher chitinase and protein production. In these conditions fusant Trichoderma HF9 in comparison with parent strains had 3-, 2.5- and 1.5-fold increase of total chitinase, specific chitinase and protein, respectively. SDS-PAGE analysis revealed that it had 9 major protein bands with up-regulation compared to parent strains. Amino acid analysis showed that protein of culture filtrate of T. harzianum, T. viride and fusant Trichoderma HF9 had 8, 6 and 10 amino acids, respectively. The results obtained suggested that fusant HF9 could be an integration of T. harzianum and T. viride through protoplast fusion.
Reference Key
balasubramanian2012appliedfusant Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors N. Balasubramanian;V. Thamil Priya;S. Gomathinayagam;D. Lalithakumari;N. Balasubramanian;V. Thamil Priya;S. Gomathinayagam;D. Lalithakumari;
Journal applied biochemistry and microbiology
Year 2012
DOI
doi:10.1134/S0003683812040035
URL
https://link.springer.com/article/10.1134/S0003683812040035
Keywords
Microbiology biochemistry medical microbiology general

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