Retroviral expression in embryonic stem cells and hematopoietic stem cells

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2000
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Abstract
Achieving long-term retroviral expression in primary cells has been problematic. De novo DNA methylation of infecting proviruses has been proposed as a major cause of this transcriptional repression. Here we report the development of a mouse stem cell virus (MSCV) long terminal repeat-based retrovir …
Reference Key
sr2000molecularretroviral Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Cherry SR;Biniszkiewicz D;van Parijs L;Baltimore D;Jaenisch R;;
Journal molecular and cellular biology
Year 2000
DOI
DOI not found
URL
http://www.ncbi.nlm.nih.gov/pubmed/11003639
Keywords
National Center for Biotechnology Information NCBI NLM MEDLINE Mice Inbred C57BL gene expression regulation animals pubmed abstract nih national institutes of health national library of medicine research support non-u.s. gov't u.s. gov't non-p.h.s. P.H.S. cell differentiation recombinant fusion proteins / genetics pmid:11003639 pmc86295 doi:10.1128/mcb.20.20.7419-7426.2000 s r cherry d biniszkiewicz r jaenisch adoptive transfer azacitidine / analogs & derivatives azacitidine / pharmacology dna (cytosine-5-)-methyltransferase 1 dna (cytosine-5-)-methyltransferases / genetics dna methylation / drug effects decitabine flow cytometry viral* / drug effects gene silencing* / drug effects genetic vectors / genetics* hematopoietic stem cells / cytology hematopoietic stem cells / drug effects hematopoietic stem cells / metabolism* hematopoietic stem cells / virology transgenic proviruses / drug effects proviruses / genetics retroviridae / drug effects retroviridae / genetics* stem cells / cytology stem cells / drug effects stem cells / metabolism* stem cells / virology terminal repeat sequences / genetics

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