Cloning and expression of African swine fever virus K205R and B602L genes inf. COLI

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ID: 109674
2018
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Abstract
A molecular cloning of African swine fever virus k205r and b602l genes in £ coli was carried out. The expression and purification conditions ensuring high yield of recombinant proteins were optimized. Dissolved recombinant proteins were purified by metal-chelate affinity chromatography using Ni-NTA-agarose («Qiagen»). Recombinant antigens are biologically safe, easier to prepare and ensure higher ELISA specificity. The purified protein yield from 100 ml off. coli culture was 1,5 mg for pK205R and 2 mg for pB602L.
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scherbakov2018cloning Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Scherbakov, A. V.;Yakovleva, A. S.;Timina, A. M.;Yakupov, M. R.;
Journal Ветеринария сегодня
Year 2018
DOI
DOI not found
URL
https://veterinary.arriah.ru/jour/article/view/191
Keywords
Veterinary medicine genetics

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