Characterization of Two Self-Sufficient Monooxygenases, CYP102A15 and CYP102A170, as Long-Chain Fatty Acid Hydroxylases.

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ID: 109640
2019
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Abstract
Self-sufficient P450s, due to their fused nature, are the most effective tools for electron transfer to activate C-H bonds. They catalyze the oxygenation of fatty acids at different omega positions. Here, two new, self-sufficient cytochrome P450s, named CYP102A15 and CYP102A170, from polar sp. PAMC 25034 and sp. PAMC 22724, respectively, were cloned and expressed in . The genes are homologues of CYP102A1 from . They catalyzed the hydroxylation of both saturated and unsaturated fatty acids ranging in length from C-C, with a moderately diverse profile compared to other members of the CYP102A subfamily. CYP102A15 exhibited the highest activity toward linoleic acid with K 15.3 μM, and CYP102A170 showed higher activity toward myristic acid with Km 17.4 μM. CYP10A170 also hydroxylated the Eicosapentaenoic acid at ω-1 position only. Various kinetic parameters of both monooxygenases were also determined.
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rimal2019characterizationjournal Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Rimal, Hemraj;Lee, Woo-Haeng;Kim, Ki-Hwa;Park, Hyun;Oh, Tae-Jin;
Journal Journal of microbiology and biotechnology
Year 2019
DOI
10.4014/jmb.1911.11048
URL
Keywords
cytochrome p450 bacillus sp. paenibacillus sp. fatty acid hydroxylation self-sufficient monooxygenase

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