Efficient production of recombinant SARS-CoV-2 spike protein using the baculovirus-silkworm system.

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ID: 109630
2020
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Ranked #40 of 66 articles by views in Biochemical and biophysical research communications

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Abstract
In the case of a new viral disease outbreak, an immediate development of virus detection kits and vaccines is required. For COVID-19, we established a rapid production procedure for SARS-CoV-2 spike protein (S protein) by using the baculovirus-silkworm expression system. The baculovirus vector-derived S proteins were successfully secreted to silkworm serum, whereas those formed insoluble structure in the larval fat body and the pupal cells. The ectodomain of S protein with the native sequence was cleaved by the host furin-protease, resulting in less recombinant protein production. The S protein modified in furin protease-target site was efficiently secreted to silkworm serum and was purified as oligomers, which showed immunoreactivity for anti-SARS-CoV-2 S2 antibody. By using the direct transfection of recombinant bacmid to silkworms, we achieved the efficient production of SARS-CoV-2 S protein as fetal bovine serum (FBS)-free system. The resultant purified S protein would be useful tools for the development of immunodetection kits, antigen for immunization for immunoglobulin production, and vaccines.
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fujita2020efficientbiochemical Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Fujita, Ryosuke;Hino, Masato;Ebihara, Takeru;Nagasato, Takumi;Masuda, Akitsu;Lee, Jae Man;Fujii, Tsuguru;Mon, Hiroaki;Kakino, Kohei;Nagai, Ryo;Tanaka, Miyu;Tonooka, Yoshino;Moriyama, Takato;Kusakabe, Takahiro;
Journal Biochemical and biophysical research communications
Year 2020
DOI
S0006-291X(20)31214-6
URL
Keywords
sars-cov-2 spike protein silkworm-bmnpv expression system

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