Universal method for direct bioconjugation of electrode surfaces by fast enzymatic polymerization.

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ID: 108429
2019
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Abstract
We report HRP-catalyzed polymerization of Tannic acid (TA) and application of the poly (Tannic acid) (p(TA)) as a versatile platform for covalent immobilization of biomolecules on various electrode surfaces based on electrochemical oxidation of the p(TA) and subsequent oxidative coupling reactions with the biomolecules. We also used this method for capturing cancer cells through a linker molecule, folic acid (FA). Furthermore, we have demonstrated that enhanced electrocatalytic activity of the p(TA)-modified surface could be used for simultaneous electrochemical determination of biologically important electroactive molecules such as ascorbic acid (AA), dopamine (DA), and uric acid (UA). This HRP-catalyzed polymerization of TA and p(TA)-mediated surface modification method can provide a simple and new framework to construct multifunctional platforms for covalent attachment of biomolecules and development of sensitive electrochemical sensing devices.
Reference Key
kumar2019universalbiosensors Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Kumar, Sumit;Haque, Al-Monsur Jiaul;Sabaté Del Río, Jonathan;Gautam, Shreedhar;Cho, Yoon-Kyoung;
Journal Biosensors & bioelectronics
Year 2019
DOI
S0956-5663(18)30971-0
URL
Keywords
bioconjugation tannic acid enzymatic polymerization electrode modification

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