Recombinant expression and molecular engineering of the keratinase from Brevibacillus parabrevis for dehairing performance.

Clicks: 375
ID: 108328
2020
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Abstract
Keratinase is capable of distinctive degradation of keratin, which provides an eco-friendly approach for keratin waste management towards sustainable development. In this study, the recombinant keratinase (KERBP) from Brevibacillus parabrevis was successfully expressed in Escherichia coli. The purified KERBP had the specific activity of 6005.3 U/mg. It showed remarkable tolerance to various surfactants and also no collagenolytic activity. However, the moderate thermal stability limited its further application. Thus, protein engineering was further adopted to improve its stability. The variants of T218S, S236C and N181D were constructed by site-directed mutagenesis and combinatorial mutagenesis. Compared with the wild type, the t at 60 °C for the variants T218S, S236C and N181D were 3.05-, 1.18- and 1-fold increase, respectively. Moreover, the double variants N181D-T218S and N181D-S236C significantly improved thermostability with 5.1 and 2.9 °C increase of T, and prolonging t at 60 °C with 4.09 and 1.54-fold, respectively. And the catalytic efficiency of the T218S and N181D-T218S variants was also significantly improved. Furthermore, the keratinase displayed favorable ability to dehair wool from skin within 7 h, which showed potential in leather dehairing. Our work contributes to a further insight into the thermostability of keratinase and offers a promising alternative for industrial leather application.
Reference Key
zhang2020recombinantjournal Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Zhang, Rong-Xian;Gong, Jin-Song;Su, Chang;Qin, Jiufu;Li, Heng;Li, Hui;Shi, Jin-Song;Xu, Zheng-Hong;
Journal journal of biotechnology
Year 2020
DOI
S0168-1656(20)30164-4
URL
Keywords
expression thermostability site-directed mutagenesis brevibacillus parabrevis keratinase

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