sp. nov., isolated from a Yellow River sample.

A novel actinobacterium, designated strain CFH S0261, was isolated from a sediment sample of the Yellow River. The taxonomic position of the strain was investigated by using a polyphasic approach. Cells of strain CFH S0261 were Gram-reaction-positive, aerobic, non-motile. Growth occurs at 15-37 °C, pH 6.0-8.0 and with 0-9.0 % (w/v) NaCl. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain CFH S0261 was a member of the genus . The 16S rRNA gene sequence similarity indicated that strain CFH S0261 is most closely related to the type strains of LC11 (98.95 %), LC2 (98.81 %) and IMSNU 22139 (98.73 %). The whole-genome of CFH S0261 showed a G+C content of 69.5 mol%. The ANI values and DDH values between CFH S0261 and the other species of the genus were found to be low (ANIb <90.61 % and DDH <53.40 %). The cell wall diamino acid in the peptidoglycan of strain CFH S0261 was -diaminopimelic acid and the whole-cell hydrolysate comprised arabinose, galactose, glucose, rhamnose and ribose. The predominant menaquinone was MK-9(H). The major cellular fatty acids were C, iso-C and iso-C. The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides and four unidentified glycolipids. On the basis of phenotypic, genotypic and phylogenetic data, strain CFH S0261 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CFH S0261 (=KCTC 39515 =CCTCC AA 2014028).