The Architecture of SARS-CoV-2 Transcriptome.

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ID: 104707
2020
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Abstract
SARS-CoV-2 is a betacoronavirus responsible for the COVID-19 pandemic. Although the SARS-CoV-2 genome was reported recently, its transcriptomic architecture is unknown. Utilizing two complementary sequencing techniques, we present a high-resolution map of the SARS-CoV-2 transcriptome and epitranscriptome. DNA nanoball sequencing shows that the transcriptome is highly complex owing to numerous discontinuous transcription events. In addition to the canonical genomic and 9 subgenomic RNAs, SARS-CoV-2 produces transcripts encoding unknown ORFs with fusion, deletion, and/or frameshift. Using nanopore direct RNA sequencing, we further find at least 41 RNA modification sites on viral transcripts, with the most frequent motif, AAGAA. Modified RNAs have shorter poly(A) tails than unmodified RNAs, suggesting a link between the modification and the 3' tail. Functional investigation of the unknown transcripts and RNA modifications discovered in this study will open new directions to our understanding of the life cycle and pathogenicity of SARS-CoV-2.
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kim2020thecell Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Kim, Dongwan;Lee, Joo-Yeon;Yang, Jeong-Sun;Kim, Jun Won;Kim, V Narry;Chang, Hyeshik;
Journal Cell
Year 2020
DOI
S0092-8674(20)30406-2
URL
Keywords
coronavirus transcriptome nanopore rna modification covid-19 sars-cov-2 direct rna sequencing discontinuous transcription epitranscriptome poly(a) tail

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