Live imaging of alterations in cellular morphology and organelles during cornification using an epidermal equivalent model.

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ID: 103543
2020
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Abstract
The stratum corneum plays a crucial role in epidermal barrier function. Various changes occur in granular cells at the uppermost stratum granulosum during cornification. To understand the temporal details of this process, we visualized the cell shape and organelles of cornifying keratinocytes in a living human epidermal equivalent model. Three-dimensional time-lapse imaging with a two-photon microscope revealed that the granular cells did not simply flatten but first temporarily expanded in thickness just before flattening during cornification. Moreover, before expansion, intracellular vesicles abruptly stopped moving, and mitochondria were depolarized. When mitochondrial morphology and quantity were assessed, granular cells with fewer, mostly punctate mitochondria tended to transition to corneocytes. Several minutes after flattening, DNA leakage from the nucleus was visualized. We also observed extension of the cell-flattening time induced by the suppression of filaggrin expression. Overall, we successfully visualized the time-course of cornification, which describes temporal relationships between alterations in the transition from granular cells to corneocytes.
Reference Key
ipponjima2020livescientific Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Ipponjima, Sari;Umino, Yuki;Nagayama, Masaharu;Denda, Mitsuhiro;
Journal Scientific reports
Year 2020
DOI
10.1038/s41598-020-62240-3
URL
Keywords
equity diversity workforce disparities genetic counseling discrimination lived experience cultural competence inclusion representation underrepresented populations

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