Copper(II)-binding equilibria in human blood.

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ID: 103098
2020
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Abstract
It has been reported that Cu(II) ions in human blood are bound mainly to serum albumin (HSA), ceruloplasmin (CP), alpha-2-macroglobulin (α2M) and His, however, data for α2M are very limited and the thermodynamics and kinetics of the copper distribution are not known. We have applied a new LC-ICP MS-based approach for direct determination of Cu(II)-binding affinities of HSA, CP and α2M in the presence of competing Cu(II)-binding reference ligands including His. The ligands affected both the rate of metal release from Cu•HSA complex and the value of K. Slow release and K = 0.90 pM was observed with nitrilotriacetic acid (NTA), whereas His showed fast release and substantially lower K = 34.7 fM (50 mM HEPES, 50 mM NaCl, pH 7.4), which was explained with formation of ternary His•Cu•HSA complex. High mM concentrations of EDTA were not able to elicit metal release from metallated CP at pH 7.4 and therefore it was impossible to determine the K value for CP. In contrast to earlier inconclusive evidence, we show that α2M does not bind Cu(II) ions. In the human blood serum ~75% of Cu(II) ions are in a nonexchangeable manner bound to CP and the rest exchangeable copper is in an equilibrium between HSA (~25%) and Cu(II)-His-Xaa ternary complexes (~0.2%).
Reference Key
kirsipuu2020copperiibindingscientific Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Kirsipuu, Tiina;Zadorožnaja, Anna;Smirnova, Julia;Friedemann, Merlin;Plitz, Thomas;Tõugu, Vello;Palumaa, Peep;
Journal Scientific reports
Year 2020
DOI
10.1038/s41598-020-62560-4
URL
Keywords
ethanol bioreactor simulation biothermodynamics syngas fermentation

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