Production of a specific monoclonal antibody for 1-naphthol based on novel hapten strategy and development of an easy-to-use ELISA in urine samples.

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ID: 102972
2020
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Abstract
1-naphthol (1-NAP) is the main metabolite of pesticide carbaryl and naphthalene, and is also a genotoxic and carcinogenic intermediate in the synthesis of organic compound, dyes, pigment and pharmaceutical industry. In this work, two novel haptens were designed and synthesized for developing a competitive indirect enzyme-linked immunosorbent assay (ciELISA) method for 1-NAP in urine samples. The assay showed a limit of detection of 2.21 ng/mL and working range from 4.02 ng/mL to 31.25 ng/mL for 1-NAP in optimized working buffer. The matrix effect of samples was eliminated via 15-fold dilution of optimized working buffer. Good average recoveries (102.4%-123.4%) with a coefficient of variation from 11.7% to 14.7% was obtained for spiked urine samples. Subsequent instrument verification test showed good correlation between the results of ciELISA and high-performance liquid chromatography. The developed ciELISA is a high-throughput tool to monitor 1-NAP in urine, which can provide technical support for the establishment of biological exposure level for the exposure to carbaryl, naphthalene and other related pollutants.
Reference Key
chen2020productionecotoxicology Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Chen, Zi-Jian;Liu, Xi-Xia;Xiao, Zhi-Li;Fu, Hui-Jun;Huang, Yu-Ping;Huang, Shu-Yi;Shen, Yu-Dong;He, Fan;Yang, Xing-Xing;Hammock, Bruce;Xu, Zhen-Lin;
Journal Ecotoxicology and environmental safety
Year 2020
DOI
S0147-6513(20)30372-9
URL
Keywords
Monoclonal antibody immunoassay 1-naphthol hapten design

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