Characterization and Functional Analysis of Two Transmembrane C-Type Lectins in Obscure Puffer ().

Clicks: 304
ID: 102260
2020
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal
Emerging

Ranked #30 of 111 articles by views in Frontiers in immunology

Most read Least read

Bar heights use a square-root scale.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
C-type lectins (CTLs) have received widespread attention in animal immune responses. In the present study, two CTLs ( and ) were identified from obscure puffer . The open reading frames of ToCTL1 and ToCTL2 were 687 and 1,380 bp, respectively. The predicted ToCTL1 and ToCTL2 proteins contained a single transmembrane region and one typical carbohydrate recognition domain (CRD). Quantitative real-time polymerase chain reaction detected and transcripts in all examined tissues, with high levels in the intestine and kidney, and their expression levels were remarkably altered upon and infection. The recombinant proteins ToCTL1-CRD and ToCTL2-CRD agglutinated the Gram-negative and Gram-positive bacteria in a Ca-dependent manner. rToCTL1-CRD and rToCTL2-CRD exhibited evident binding activities against seven kinds of bacteria and polysaccharides (lipopolysaccharide and peptidoglycan) in a Ca-independent manner. Moreover, rToCTL1-CRD and rToCTL2-CRD could inhibit the growth of four types of bacteria . These findings collectively demonstrated that ToCTL1 and ToCTL2 could be involved in host defense against bacterial infection in .
Reference Key
huang2020characterizationfrontiers Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Huang, Ying;Shi, Yan;Hu, Sufei;Wu, Ting;Zhao, Zhe;
Journal Frontiers in immunology
Year 2020
DOI
10.3389/fimmu.2020.00436
URL
Keywords
recombinant protein c-type lectin expression analysis takifugu obscurus antibacterial response transmembrane region

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.