Isolation, Genomic and Metabolomic Characterization of VITAKN with Quorum Sensing Inhibitory Activity from Southern India.

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ID: 101062
2020
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Abstract
are among the most promising genera in terms of production ability to biosynthesize a variety of bioactive secondary metabolites with pharmaceutical interest. Coinciding with the increase in genomic sequencing of these bacteria, mining of their genomes for biosynthetic gene clusters (BGCs) has become a routine component of natural product discovery. Herein, we describe the isolation and characterization of a VITAKN with quorum sensing inhibitory (QSI) activity that was isolated from southern coastal part of India. The nearly complete genome consists of 8,621,231bp with a GC content of 72.2%. Sequence similarity networks of the BGCs detected from this strain against the Minimum Information about a Biosynthetic Gene Cluster (MIBiG) database and 3365 BGCs predicted by antiSMASH analysis of publicly available complete genomes were generated through the BiG-SCAPE-CORASON platform to evaluate its biosynthetic novelty. Crude extract analysis using high-performance liquid chromatography connected to high resolution tandem mass spectrometry (HPLC-HRMS/MS) and dereplication through the Global Natural Product Social Molecular Networking (GNPS) online workflow resulted in the identification of cyclic dipeptides (2, 5-diketopiperazines, DKPs) in the extract, which are known to possess QSI activity. Our results highlight the potential of genome mining coupled with LC-HRMS/MS and in silico tools (GNPS) as a valid approach for the discovery of novel QSI lead compounds. This study also provides the biosynthetic diversity of BGCs and an assessment of the predicted chemical space yet to be discovered.
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ishaque2020isolationmicroorganisms Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Ishaque, Nabila Mohammed;Burgsdorf, Ilia;Limlingan Malit, Jessie James;Saha, Subhasish;Teta, Roberta;Ewe, Daniela;Kannabiran, Krishnan;Hrouzek, Pavel;Steindler, Laura;Costantino, Valeria;Saurav, Kumar;
Journal Microorganisms
Year 2020
DOI
E121
URL
Keywords
actinobacteria lc-hrms natural product biosynthetic gene clusters (bgcs) cyclic dipeptides (2,5-diketopiperazines, dkps) global natural product social networking (gnps) quorum sensing inhibition (qsi)

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