A comparison of molecular markers to detect Lutzomyia longipalpis naturally infected with Leishmania (Leishmania) infantum

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ID: 100892
2014
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Abstract
The aim of the present study was to detect natural infection by Leishmania (Leishmania) infantum in Lutzomyia longipalpis captured in Barcarena, state of Pará, Brazil, through the use of three primer sets. With this approach, it is unnecessary to previously dissect the sandfly specimens. DNA of 280 Lu. longipalpis female specimens were extracted from the whole insects. PCR primers for kinetoplast minicircle DNA (kDNA), the mini-exon gene and the small subunit ribosomal RNA (SSU-rRNA) gene of Leishmania were used, generating fragments of 400 bp, 780 bp and 603 bp, respectively. Infection by the parasite was found with the kDNA primer in 8.6% of the cases, with the mini-exon gene primer in 7.1% of the cases and with the SSU-rRNA gene primer in 5.3% of the cases. These data show the importance of polymerase chain reaction as a tool for investigating the molecular epidemiology of visceral leishmaniasis by estimating the risk of disease transmission in endemic areas, with the kDNA primer representing the most reliable marker for the parasite.
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freitaslidani2014amemorias Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Freitas-Lidani, Kárita Cláudia;Messias-Reason, Iara J de;Ishikawa, Edna Aoba Y;
Journal memórias do instituto oswaldo cruz
Year 2014
DOI
DOI not found
URL
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000400442&lng=en&tlng=en
Keywords
Microbiology Medicine (General) Medicine Science arctic medicine. tropical medicine visceral leishmaniasis tropical medicinemicrobiology

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