Enzymatic Conversion of RBCs by -N-Acetylgalactosaminidase from Spirosoma linguale
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ID: 10033
2019
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Abstract
Spirosoma linguale is a free-living nonpathogenic organism. Like many other bacteria, S. linguale produces a cell-associated α-N-acetylgalactosaminidase. This work was undertaken to elucidate the nature of this activity. The recombinant enzyme was produced, purified, and examined for biochemical attributes. The purified enzyme was ~50 kDa active as a homodimer in solution. It catalyzed hydrolysis of α-N-acetylgalactosamine at pH 7. Calculated was 1.1 mM with of 173 s−1. The described enzyme belongs to the GH109 family.
| Reference Key |
j2019enzymaticenzyme
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| Authors | Malinski, Thomas J.;Singh, Harkewal;Malinski, Thomas J.;Singh, Harkewal; |
| Journal | enzyme research |
| Year | 2019 |
| DOI |
10.1155/2019/6972835
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| URL | |
| Keywords | Keywords not found |
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