Enzymatic Conversion of RBCs by -N-Acetylgalactosaminidase from Spirosoma linguale

Clicks: 237
ID: 10033
2019
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Abstract
Spirosoma linguale is a free-living nonpathogenic organism. Like many other bacteria, S. linguale produces a cell-associated α-N-acetylgalactosaminidase. This work was undertaken to elucidate the nature of this activity. The recombinant enzyme was produced, purified, and examined for biochemical attributes. The purified enzyme was ~50 kDa active as a homodimer in solution. It catalyzed hydrolysis of α-N-acetylgalactosamine at pH 7. Calculated was 1.1 mM with of 173 s−1. The described enzyme belongs to the GH109 family.
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j2019enzymaticenzyme Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Malinski, Thomas J.;Singh, Harkewal;Malinski, Thomas J.;Singh, Harkewal;
Journal enzyme research
Year 2019
DOI
10.1155/2019/6972835
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