Developing Single Nucleotide Polymorphisms for Identification of Cod Products by RAD-Seq.

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ID: 100313
2020
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Abstract
The increase in the rate of seafood fraud, particularly in the expensive fishes, forces us to verify the identity of marine products. Meanwhile, the definition of cod lacks consistency at the international level, as few standards and effective application methods are capable of accurately detecting cod species. Genetic fingerprinting is important for both certifying authenticity and traceability of fish species. In this study, we developed a method that combines DNA barcoding and the restriction-site associated DNA sequencing (RAD-Seq) approach for the identification of cod products. We first obtained 6941 high-quality single nucleotide polymorphism (SNP)s from 65.6 gigabases (Gb) of RAD-Seq raw data, and two sequences that contain SNPs were finally used to successfully identify three different cod product species, which are Atlantic cod (), Greenland turbot (), and Patagonian toothfish (). This SNP-based method will help us to identify the products, which are sold under the name of "Xue Yu" (Cod) in China, and works in parallel with existing fish identification techniques to establish an efficient framework to detect and prevent fraud at all points of the seafood supply chain.
Reference Key
jiang2020developinganimals Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Jiang, Shoujia;Ma, Xingyu;Li, Tao;Zhu, Changqing;You, Xinxin;
Journal animals
Year 2020
DOI
E423
URL
Keywords
snps species identification rad-seq cod products

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