Changes in the abundance and community composition of different nitrogen cycling groups in response to fumigation with 1,3-dichloropropene.

Abstract

The fumigant 1,3-dichloropropene (1,3-D) is widely-used to control pathogenic bacteria, fungi, nematodes and insects in soil before a crop is planted. Although fumigants in general have been reported to have a 'fertilizer effect' in the soil by increasing nitrogen availability, little is known of how a specific fumigant such as 1,3-D affects available nitrogen. This study used real-time quantitative PCR (qPCR) and 16S rRNA gene amplicon sequencing techniques to investigate the effects of 1,3-D on microorganisms involved in nitrogen cycling that were present in 2 soils: Jiangxi lateritic red soil and Beijing fluvo-aquic soil. The fumigant 1,3-D temporarily decreased the abundance of 11 functional genes involved in nitrogen-fixing, nitrification and denitrification in both soil types. Different nitrogen cycling groups recovered to the unfumigated level in various incubation phases. Microorganisms containing nifH, nxrB, napA and qnorB genes were most vulnerable to 1,3-D fumigation. However, a stronger and longer inhibition effect of 1,3-D on these 11 functional genes was observed in Jiangxi soil than in Beijing soil. At the same time, the abundance of nifH, AOBamoA, nirS, qnorB and cnorB genes was significantly increased 59 days after 1,3-D fumigation. Fumigation with 1,3-D significantly reduced the nitrogen-fixing bacteria Azospirillum and Paenibacillus; the nitrifiers Nitrosomonas and Nitrospira; and the denitrifiers Pseudomonas, Paracoccus and Sphingomonas. Conversely, fumigation with 1,3-D increased the nitrogen-fixing bacteria Bradyrhizobium and Rhizobium; the nitrification bacteria Nitrosospira and Nitrolancea; and the denitrification bacteria Sphingobium, Alcanivorax, Bacillus, Streptomyces and Aeromonas. Fumigation with 1,3-D therefore caused significant shifts in the species composition and number of microbes directly involved in nitrogen cycling in the short-term. These results contribute toward a better understanding of the impact of 1,3-D fumigation on various types of soil nitrogen-cycling groups.