Application of Magnetic Resonance Imaging Molecular Probe in the Study of Pluripotent Stem Cell-derived Neural Stem Cells for the Treatment of Post-traumatic Paralysis of Cerebral Infarction.

The paper studied the feasibility and efficacy of magnetic resonance imaging molecular probe application and pluripotent stem cell-derived neural stem cell transplantation for the treatment of hind limb paralysis in mice with cerebral infarction. This paper used adult mice as experimental objects to establish a model of middle cerebral artery infarction and stimulate hindlimb reactions. After the model was successfully established, the mice were first divided into an experimental group and a control group, with 25 mice in each group. Cultured neural cells were obtained from the cerebral cortex and hippocampus of a mouse 15 days pregnant to prepare pluripotent stem cells. Pluripotent stem cell-derived neural stem cells were identified by positive expression of nestin. The experimental group was injected with 1μL of neural stem cell suspension through the tail vein, and the control group was injected with 1μL of saline through the tail vein. 1 day, 3 days, 7 days, 14 days and 28 days after transplantation, the neurological function of mice in each group was scored according to Garcia 18 subscale. Finally, the differentiation, migration, and integration of pluripotent stem cell-derived neural stem cells after transplantation were observed using a magnetic resonance imaging molecular probe method. The results showed that the neurological function scores of the ischemic transplantation group were significantly higher than those of the control group, and the results were different, and the results were significantly different (P<0.05). Through research, it was found that after transplantation of pluripotent stem cell-derived neural stem cells, the transplanted cells migrated and differentiated around the body at 28 days, and participated in angiogenesis, and the blood vessels in the infarcted area were obviously proliferated. The neural stem cells cultured in vitro were transplanted to the small infarction after cerebral infarction. In rats, it plays a positive role in the repair of nerve function in mice with cerebral infarction. Neural stem cells cultured in vitro can survive, migrate and differentiate in the brain tissue of mouse ischemic models, and play a positive role in the repair of neurological function in mice with cerebral infarction. Magnetic resonance imaging molecular probes have a good adjuvant effect on the use of pluripotent stem cell-derived neural stem cells to treat hindlimb paralysis in mice with cerebral infarction.