Prevention of Enamel Softening by Rinsing with a Calcium Solution before Dental Erosion.

This in situ study aimed to evaluate whether rinsing with a calcium-containing solution prior to an erosive attack reduces the softening of enamel.A total of 240 bovine enamel samples with determined baseline surface microhardness (KHN) were allocated to 5 runs in which each of the 12 volunteers performed the following experiment: 4 enamel samples were inserted in a custom-made intraoral appliance and carried in the mouth (upper jaw) for 30 min before each volunteer either rinsed his mouth for 60 s with a fluoride- and stannous ion-containing dental erosion protection mouth rinse as positive control (run 1), milk (run 2), a solution prepared from a 500-mg calcium effervescent tablet dissolved in 100 mL (run 3) or 200 mL (run 4) water, or did not perform any rinsing with a test solution before the erosive attack (run 5, negative control). To simulate the erosive attack, volunteers rinsed their mouth with a commercial soft drink (Sprite Zero) for 60 s and afterwards with water to stop the erosive process. Finally, surface microhardness was measured again and hardness loss (ΔKHN) calculated. A mixed effect model was fitted to the data set to investigate whether the different runs showed differences with respect to ΔKHN.No significant difference in softening of enamel (mean of ΔKHN; lower confidence level/upper confidence level) was observed between the negative control run 5 (50.7; 60.8/40.6), run 2 (50.7; 60.8/40.6), run 3 (38.7; 48.8/28.6) and run 4 (40.7; 50.8/30.6) (p > 0.05, respectively). Enamel softening in the positive control run 1 (25.4; 35.6/15.3) was significantly lower compared to the softening in run 5 (p < 0.001). No significant difference was observed between run 1 and run 3 (p = 0.09).Other than the fluoride- and stannous ion-containing dental erosion protection mouth rinse, none of the investigated calcium-containing solutions is able to significantly reduce erosion-induced softening of enamel.