Early Administration of Glutamine Protects Cardiomyocytes from Post-Cardiac Arrest Acidosis

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ID: 77330
2016
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Abstract
Postcardiac arrest acidosis can decrease survival. Effective medications without adverse side effects are still not well characterized. We aimed to analyze whether early administration of glutamine could improve survival and protect cardiomyocytes from postcardiac arrest acidosis using animal and cell models. Forty Wistar rats with postcardiac arrest acidosis (blood pH < 7.2) were included. They were divided into study (500 mg/kg L-alanyl-L-glutamine, n=20) and control (normal saline, n=20) groups. Each of the rats received resuscitation. The outcomes were compared between the two groups. In addition, cardiomyocytes derived from human induced pluripotent stem cells were exposed to HBSS with different pH levels (7.3 or 6.5) or to culture medium (control). Apoptosis-related markers and beating function were analyzed. We found that the duration of survival was significantly longer in the study group (p<0.05). In addition, in pH 6.5 or pH 7.3 HBSS buffer, the expression levels of cell stress (p53) and apoptosis (caspase-3, Bcl-xL) markers were significantly lower in cardiomyocytes treated with 50 mM L-glutamine than those without L-glutamine (RT-PCR). L-glutamine also increased the beating function of cardiomyocytes, especially at the lower pH level (6.5). More importantly, glutamine decreased cardiomyocyte apoptosis and increased these cells’ beating function at a low pH level.
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lin2016earlybiomed Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Lin, Yan-Ren;Li, Chao-Jui;Syu, Shih-Han;Wen, Cheng-Hao;Buddhakosai, Waradee;Wu, Han-Ping;Chen, Cheng Hsu;Lu, Huai-En;Chen, Wen-Liang;
Journal BioMed research international
Year 2016
DOI DOI not found
URL
http://dx.doi.org/10.1155/2016/2106342
Keywords
chemistry Microbiology Medicine Chemical technology Biotechnology Technology Computer applications to medicine. Medical informatics Science (General) Science physics

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