Gene expression in response to Cotton Leaf Curl Virus infection in Gossypium hirsutum under variable environmental conditions
Clicks: 228
ID: 77000
2017
Cotton Leaf Curl Disease (CLCuD) is one of the threatening constrains of
cotton production in Pakistan for which no adequate remedy is available until
now. Local variety of Gossypium hirsutum (FH-142) was grown in field and
infected naturally by CLCuV under variable range of temperature and humidity.
Plants showed thickening of veins in lower leaf surface at 34°C and 60%
relative humidity at 15days post infection (dpi) and curling of leaf margins
at 33°C with 58% relative humidity at 30dpi. Remarkable leaf darkening was
observed with reduced boll formation at 45dpi at 26°C and 41% relative
humidity. Enation developed, severe thickening and curling of leaves
intensified and plants showed dwarf growth at 60dpi at 24°C with 52% relative
humidity. PCR amplification of Rep associated gene confirmed the presence of
CLCuD-associated begomovirus in the infected samples. Quantitative RT-PCR
confirmed the amplification and differential expression of a number of
pathogen stress responsive genes at different levels of temperature and
humidity. This observation predicts that Cotton Leaf Curl Virus (CLCuV)
interacts with several host genes that are upregulated to make plants
susceptible or suppress other genes to overcome host defense responses.
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| Authors | Iqra, Rehman;Beenish, Aftab;Muhammad, Bilal Sarwar;Bushra, Rashid;Qurban, Ali;Muhammad, Umair Majid;Sameera, Hassan;Muhammad, Azam Ali;Idrees, Ahmad Nasir;Muhammad, Saleem Haider;Tayyab, Husnain; |
| Journal | genetika |
| Year | 2017 |
| DOI | DOI not found |
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