Experimental Comparison in Sensing Breast Cancer Mutations by Signal ON and Signal OFF Paper-Based Electroanalytical Strips.

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ID: 74820
2019
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Abstract
The development of paper-based electroanalytical strips as powerful diagnostic tools has gained a lot of attention within the sensor community. In particular, the detection of nucleic acids in complex matrices represents a trending topic, especially when focused towards the development of emerging technologies, such as liquid biopsy. The DNA-based biosensors have been largely applied in this direction and, currently, there are two main approaches based on target/probe hybridization re-ported in literature, namely Signal ON and Signal OFF. In this technical note, the two approaches are evaluated in combina-tion with paper-based electrodes, using a single strand DNA relative to H1047R (A3140G) missense mutation in exon 20 in breast cancer as the model target. A detailed comparison among the analytical performances, detection protocol, and cost as-sociated with the two systems is provided, highlighting the advantages and drawbacks depending on the application. The present work is aimed to a wide audience, particularly for those in the field of point-of-care, and it is intended to provide the know-how to manage with the design and development stages, and to optimize the platform for the sensing of nucleic acids using a paper-based detection method.
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cinti2019experimentalanalytical Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Cinti, Stefano;Cinotti, Giulia;Parolo, Claudio;Nguyen, Emily Phuong;Caratelli, Veronica;Moscone, Danila;Arduini, Fabiana;Merkoçi, Arben;
Journal Analytical chemistry
Year 2019
DOI 10.1021/acs.analchem.9b02560
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Keywords
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