Methotrexate Restores CD73 Expression on Th1.17 in Rheumatoid Arthritis and Psoriatic Arthritis Patients and May Contribute to Its Anti-Inflammatory Effect through Ado Production.

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2019
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Abstract
Th1.17 are highly polyfunctional, potentially harmful CD4 effector T cells (Teff) through IFN-γ and IL-17A coproduction. Th1.17 take part in the pathophysiology of rheumatoid arthritis (RA) and psoriatic arthritis (PsA), in which their hyper activation results in part from defects in negative regulation mechanisms. We recently demonstrated that the ecto-nucleotidase CD73 delineates a Th1.17-enriched Teff population and acts as an endogenous regulatory mechanism. Because Methotrexate (MTX), used as first line treatment of RA and PsA, increases extracellular concentrations of AMP and immunosuppressive adenosine, we investigated the modulation of CD73 by MTX treatment on Teff in RA/PsA patients.In a prospective cohort of 26 RA and 15 PsA patients before or under MTX treatment, we evaluated CD73 expression on blood Teff subsets, their cytokine production and AMPase functions.We showed a decreased CD73 expression on Th1.17 and Th1 in untreated patients compared to healthy donors that was partly restored under MTX. This decrease in untreated patients leads to a halved Ado production by Th1.17 cells. CD73 Teff remained functional under MTX treatment, but their CD73 re-expression may contribute to control their activation.Our study unveils uncovered mode of action of MTX on Teff subsets modulation and in the adenosine-dependent termination of inflammation in RA and PsA.
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Authors Bossennec, Marion;Rodriguez, Céline;Hubert, Margaux;Di-Roio, Anthony;Machon, Christelle;Guitton, Jérôme;Battiston-Montagne, Priscilla;Couturier, Mathilde;Marotte, Hubert;Caux, Christophe;Coury, Fabienne;Ménétrier-Caux, Christine;
Journal journal of clinical medicine
Year 2019
DOI E1859
URL
Keywords
Rheumatoid Arthritis regulation psoriatic arthritis methotrexate adenosine ifn-γ, cd73 il-17a th1.17

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