High-performance liquid chromatographic quantification of 4-methylumbelliferyl-beta-D-glucuronide as a probe for human beta-glucuronidase activity in tissue homogenates.
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ID: 70559
1996
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Abstract
An internally standardized HPLC method to determine the concentration of 4-methylumbelliferone liberated from 4-methylumbelliferyl-beta-D-glucuronide by human beta-glucuronidase was developed. The assay allows the precise and rapid measurement of specific enzyme activity in human tissue homogenates. Without prior extraction the incubation mixture can be separated using a C8 column followed by fluorescence detection. The assay showed good accuracy and precision with a detection limit of 20 nM and a limit of quantification of 167 nM. The suitability of the method was shown in enzyme kinetic experiments with human liver homogenates.| Reference Key |
sperker1996highperformancejournal
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| Authors | Sperker, B;Schick, M;Kroemer, H K; |
| Journal | journal of chromatography b, biomedical applications |
| Year | 1996 |
| DOI | DOI not found |
| URL | URL not found |
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