Investigating the influence of iron on Campylobacter jejuni transcriptome in response to acid stress.

The capacity of C. jejuni to survive acid and capture iron is a requirement for C. jejuni to colonize host and cause infection. Herein, we aimed to characterize the influence of iron on Campylobacter acid response. The capacity of C. jejuni to survive acid stress was greatly enhanced in presence of iron. Moreover, the acid stimulon of C. jejuni under iron-enriched condition was investigated using the microarray approach. A total of 211 genes were differentially expressed in C. jejuni. Differentially expressed genes were included in 21 functional groups that control Campylobacter physiology. C. jejuni induced expression of many genes that were previously shown to be important for Campylobacter acid survival such as flagella biogenesis genes and genes involved in cell envelope biogenesis. The microarray results were validated using RT-qPCR where there was a great similarity in data obtained by both techniques. Finally, comparative analysis with previous studies showed that acid exposure induced expression of many genes in C. jejuni that were not detected in other studies such as genes encoding for the heat shock proteins GroEL and GroES. Current data could help us understand the mechanism of C. jejuni acid survival and consequently overcome infection by this enteric pathogen.