Novel Liposomal Azido Mannosamine Lipids on Metabolic Cell Labelling and Imaging via Cu-free Click Chemistry.

Comparing with the popular Ac4ManNAz applied on cell labelling via Cu-free click chemistry, two novel azido mannosamine lipids with C6 and C12 ester on anomeric hydroxyl group were prepared and encapsulated in liposome delivery system, which enhanced chemical stabilities and showed good cell-metabolizable labelling efficiency on MDA-MB-231 cells with strong fluorescene after the treatment of DBCO-Cy5 by triazole formation via click chemistry.