Identification and Analysis of Chemical Constituents and Rat Serum Metabolites in Using UPLC-Q-TOF/MS Combined with Multiple Data-Processing Approaches.

is a dry whole grass of Thunb.; it has been extensively used to anti-inflammatory, antioxidant, and antimicrobial actions and inhibits acetylcholinesterase activity. However, it lacks further compounds research of in and in . In this work, a rapid method was established using the ultra high performance liquid chromatography with quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF/MS) combined with multiple data-processing approaches for compounds analysis of in and in . Finally, 30 peaks were characterized in 75% ethanol extract of and 17 peaks were characterized in rat plasma that including 12 prototype compounds and 5 metabolites. Methylation and demethylation are the main transformation reactions of in rat serum. This work could be helpful for understanding the complex compounds of and further analyzing the pharmacological studies of active compounds.