Fast, Sensitive and Specific Detection of and its Variants Using One-Step Real-Time Reverse-Transcription Polymerase Chain Reaction Assay.
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2019
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Abstract
Genetic variants of Thailand orthohantavirus (THAIV) have been recently reported from rodents in South-East Asia and in islands from the South-West part of the Indian Ocean. In order to detect THAIV and its variants, we developed a sensitive and specific real-time RT-PCR targeting the S segment. Our assay was developed in two different RT-PCR systems that gave similar results in terms of sensitivity. Moreover, our results demonstrated a specificity of 100%.Reference Key |
raharinosy2019fastviruses
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Authors | Raharinosy, Vololoniaina;Heraud, Jean-Michel;Rabemananjara, Harinirina Aina;Telfer, Sandra;Rakoto, Danielle Aurore Doll;Filippone, Claudia;Reynes, Jean-Marc; |
Journal | Viruses |
Year | 2019 |
DOI | E718 |
URL | |
Keywords | Keywords not found |
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