Prediction of bovine ovulation by a rapid radioimmunoassay for plasma LH
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1983
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Abstract
A commonly used and specific plasma LH radioimmunoassay was modified to provide reliable results for cow LH concentrations in blood within 4-5 h. Blood samples were collected at 2- or 4-h intervals from at least 24 h before the expected oestrus until after its onset. Thereafter, samples were collected at intervals of 2 or 4 days until the next oestrus. For the assay, the antigen-antibody reaction took place during a 2-h incubation at 37 degrees C. The immune complex was precipitated by addition of rabbit anti-gamma-globulin and polyethylene glycol followed by an incubation at 22 degrees C for 30 min. The coefficient of variation between this assay and the standard assay was less than 15%. From a study of 25 cycles from 12 animals, we obtained intervals of congruent to 27.3 h between the beginning of the LH peak and ovulation and congruent to 17.5 h between the end of the peak and ovulation. This modified technique can be used to predict rapidly and precisely the time of ovulation in cattle.| Reference Key |
lambert1983reproductionprediction
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| Authors | C. Bernard,J. P. Valet,R. Beland,R. D. Lambert;C. Bernard;J. P. Valet;R. Beland;R. D. Lambert; |
| Journal | reproduction (cambridge, england) |
| Year | 1983 |
| DOI | 10.1530/jrf.0.0680425 |
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