Optimal conditions for protein synthesis in incubated slices of rat brain

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ID: 270232
1975
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Abstract
Optimal conditions for protein synthesis in incubated slices of rat brain were determined to be: thickness 0.3 mm under air (for newborn 0.4-0.6 mm) or 0.5 mm under oxygen; temperature, 35-36 degrees C; hepes (N-2-hydroxyethylpiperazine-N'-2-ethane sulfonic acid) buffer; K+, 6-8 mM; Ca2+, 2-3 mM. Th ā€¦
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Authors Dunlop DS;van Elden W;Lajtha A;;
Journal Brain research
Year 1975
DOI DOI not found
URL
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=241463
Keywords
National Center for Biotechnology Information NCBI NLM MEDLINE Depression animals pubmed abstract nih national institutes of health national library of medicine research support u.s. gov't male Comparative Study P.H.S. Rats Temperature in vitro techniques kinetics hydrogen-ion concentration amino acids / metabolism brain / metabolism* chemical buffers calcium / pharmacology magnesium / pharmacology potassium / pharmacology pmid:241463 doi:10.1016/0006-8993(75)90031-1 d s dunlop w van elden a lajtha nerve tissue proteins / biosynthesis* ouabain / pharmacology sodium / pharmacology stimulation

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