Hybrid Bionanocomposites from Spent Hen Proteins.

Spent hens, a poultry by-product, have little economic value for processing and mostly end up in landfills. However, there are concerns over disposal of spent hens; therefore, it is pertinent to find out alternative uses that are environmentally sound. On the other hand, single-use plastic packaging is leading to a global environmental crisis. In this study, proteins were extracted from spent hen, plasticized, and processed into films by compression molding. The hybrid bionanocomposite films were successfully prepared using glycerol as a plasticizer, chitosan as a cross-linker, and varying concentrations of nanoclay as a nanoreinforcement. The effects of nanoreinforcements, plasticization, and cross-linking were then evaluated on thermal, mechanical, and barrier properties of the prepared bionanocomposite films. Various concentrations of nanoclay and chitosan were dispersed in the protein matrix. However, with the same plasticizer loading, the optimum addition of chitosan and nanoclay led to almost twofold increase in the mechanical strength, compared to neat protein films. The results indicated that at optimal conditions, a good intercalation and/or exfoliation of the protein biopolymers into clay interlayer galleries was observed leading to improved thermal, thermomechanical, and barrier properties. These hybrid bionanocomposite films have great future potential to be used in packaging and other applications.