urease activity protection with edta against nanoparticles (fe2o3 and fe3o4) inactivation

Abstract

In this study the effects of Fe2O3 and Fe3O4 magnetic nanoparticles and EDTA on urease activity was investigated. The effect of nano-Fe2O3 and nano-Fe3O4 on urease activity were investigated. Urease activity was studies by UV-Vis spectrophotometry at 40 °C at pH = 7.2 using sodium phosphate as buffer. Measurements were carried out using 0.075 mg/ml of urease and a range of nano-Fe2O3 and nano-Fe3O4 concentrations between 0.002-0.006 mg/ml. It was found that by increasing the concentration of nano-Fe2O3 and nano-Fe3O4, urease activity will be decreased. On the other hand, nano-Fe2O3 and nano-Fe3O4 act as non competitive inhibitor for urease. Urease protection studies were corried out by using different concentration of EDTA (0.004-0.008 mg/ml). It was shown by increasing the concentration of EDTA, the activity of enzyme increased.