produção, purificação, clonagem e aplicação de enzimas líticas production, purification, cloning and application of lytic enzymes

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ID: 145010
2005
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Lytic enzymes such as beta-1,3 glucanases, proteases and chitinases are able to hydrolyse, respectively, beta-1,3 glucans, mannoproteins and chitin, as well as the cell walls of many yeast species. Lytic enzymes are useful in a great variety of applications including the preparation of protoplasts; the extraction of proteins, enzymes, pigments and functional carbohydrates; pre-treatment for the mechanical rupture of cells; degradation of residual yeast cell mass for the preparation of animal feed; analysis of the yeast cell wall structure and composition; study of the yeast cell wall synthesis and the control of pathogenic fungi. This review presents the most important aspects with respect to lytic enzymes, especially their production, purification, cloning and application.
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Authors ;Luciana Francisco Fleuri;Hélia Harumi Sato
Journal greece and rome
Year 2005
DOI 10.1590/S0100-40422005000500026
URL
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422005000500026
Keywords
chitinaseschemistry

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