streptococcal endo-β-n-acetylglucosaminidase suppresses antibody-mediated inflammation in vivo
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ID: 134336
2018
Endo-β-N-acetylglucosaminidase (EndoS) is a family 18 glycosyl hydrolase secreted by Streptococcus pyogenes. Recombinant EndoS hydrolyzes the β-1,4-di-N-acetylchitobiose core of the N-linked complex type glycan on the asparagine 297 of the γ-chains of IgG. Here, we report that EndoS and IgG hydrolyzed by EndoS induced suppression of local immune complex (IC)-mediated arthritis. A small amount (1 µg given i.v. to a mouse) of EndoS was sufficient to inhibit IgG-mediated arthritis in mice. The presence of EndoS disturbed larger IC lattice formation both in vitro and in vivo, as visualized with anti-C3b staining. Neither complement binding in vitro nor antigen-antibody binding per se were affected. Thus, EndoS could potentially be used for treating patients with IC-mediated pathology.
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nandakumar2018frontiersstreptococcal
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| Authors | ;Kutty Selva Nandakumar;Kutty Selva Nandakumar;Mattias Collin;Kaisa E. Happonen;Kaisa E. Happonen;Susanna L. Lundström;Allyson M. Croxford;Bingze Xu;Roman A. Zubarev;Merrill J. Rowley;Anna M. Blom;Christian Kjellman;Rikard Holmdahl;Rikard Holmdahl |
| Journal | sudebno-meditsinskaia ekspertiza |
| Year | 2018 |
| DOI | 10.3389/fimmu.2018.01623 |
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