Quantitative fluorescence in situ hybridization of Bifidobacterium spp. with genus-specific 16S rRNA-targeted probes and its application in fecal samples

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ID: 119658
1995
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Abstract
Three 16S rRNA hybridization probes were developed and tested for genus-specific detection of Bifidobacterium species in the human fecal flora. Variable regions V2, V4, and V8 of the 16S rRNA contained sequences unique to this genus and proved applicable as target sites for oligodeoxynucleotide prob ā€¦
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Authors Langendijk PS;Schut F;Jansen GJ;Raangs GC;Kamphuis GR;Wilkinson MH;Welling GW;;
Journal Applied and environmental microbiology
Year 1995
DOI DOI not found
URL
http://www.ncbi.nlm.nih.gov/pubmed/7487040
Keywords
National Center for Biotechnology Information NCBI NLM MEDLINE RNA humans pubmed abstract nih national institutes of health national library of medicine DNA Ribosomal Base Sequence Molecular Sequence Data feces / microbiology 16s / genetics* in situ hybridization species specificity bifidobacterium / classification bifidobacterium / isolation & purification bacterial / genetics 16s / isolation & purification bacterial / isolation & purification bacterial / genetics* fluorescence / methods* pmid:7487040 pmc167584 doi:10.1128/aem.61.8.3069-3075.1995 p s langendijk f schut g w welling bifidobacterium / genetics* dna probes / genetics

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