Amplification of cDNA ends based on template-switching effect and step-out PCR
Clicks: 216
ID: 116318
1999
A new method for amplifying cDNA ends is described which requires only first-strand cDNA synthesis and a single PCR to generate a correct product with very low or no background. The method can be successfully applied to total RNA as well as poly A+ RNA. The same first-strand cDNA can be used to ampl …
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m1999nucleicamplification
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| Authors | Matz M;Shagin D;Bogdanova E;Britanova O;Lukyanov S;Diatchenko L;Chenchik A;; |
| Journal | Nucleic Acids Research |
| Year | 1999 |
| DOI | DOI not found |
| URL | |
| Keywords |
National Center for Biotechnology Information
NCBI
NLM
MEDLINE
genetic
pubmed abstract
nih
national institutes of health
national library of medicine
models
research support
non-u.s. gov't
DNA
Polymerase Chain Reaction / methods*
pmid:10037822
pmc148354
doi:10.1093/nar/27.6.1558
m matz
d shagin
a chenchik
complementary / genetics*
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