Amplification of cDNA ends based on template-switching effect and step-out PCR.

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ID: 112938
1999
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Abstract
A new method for amplifying cDNA ends is described which requires only first-strand cDNA synthesis and a single PCR to generate a correct product with very low or no background. The method can be successfully applied to total RNA as well as poly A+ RNA. ...
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chenchik1999nucleicamplification Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors M Matz, D Shagin, E Bogdanova, O Britanova, S Lukyanov, L Diatchenko, A Chenchik;M Matz;D Shagin;E Bogdanova;O Britanova;S Lukyanov;L Diatchenko;A Chenchik;
Journal Nucleic Acids Research
Year 1999
DOI 10.1093/nar/27.6.1558
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