Identification of nonpoint sources of fecal pollution in coastal waters by using host-specific 16S ribosomal DNA genetic markers from fecal anaerobes

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Abstract
We describe a new PCR-based method for distinguishing human and cow fecal contamination in coastal waters without culturing indicator organisms, and we show that the method can be used to track bacterial marker sequences in complex environments. We identified two human-specific genetic markers and f ā€¦
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Authors Bernhard AE;Field KG;;
Journal Applied and environmental microbiology
Year 2000
DOI DOI not found
URL
http://www.ncbi.nlm.nih.gov/pubmed/10742246
Keywords
National Center for Biotechnology Information NCBI NLM MEDLINE RNA bacteria animals humans pubmed abstract nih national institutes of health national library of medicine research support non-u.s. gov't u.s. gov't non-p.h.s. adult Child DNA Ribosomal Sequence Analysis polymerase chain reaction Feces / microbiology* Molecular Sequence Data Phylogeny Genetic Markers Polymorphism cattle bifidobacterium / genetics 16s / genetics* bacteroides / isolation & purification species specificity pmid:10742246 pmc92027 doi:10.1128/aem.66.4.1587-1594.2000 a e bernhard k g field anaerobic / classification anaerobic / genetics anaerobic / isolation & purification* bacteroides / classification bacteroides / genetics bifidobacterium / classification bifidobacterium / isolation & purification dna restriction enzymes / metabolism bacterial / genetics ribosomal / genetics* restriction fragment length prevotella / classification prevotella / genetics prevotella / isolation & purification water microbiology* water pollution*

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